Primary: Anti- ANKRD33B at 1/1000 dilution
Primary: Anti- ANKRD33B at 1/1000 dilution
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
