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phospho-CK II beta (Ser209) Polyclonal Antibody

货号: BS65406

价格：￥ ——

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS65406

Product Name :phospho-CK II beta (Ser209) Polyclonal Antibody

Swiss-Prot :P67871

Host :Rabbit

Reactivity :Human,Mouse,Rat,Pig,Cow,

Applications :IHC-P,

Application_all :IHC-P=1:100-500

Background :This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :phospho-CK II beta (Ser209) Polyclonal Antibody detects endogenous levels of CK II beta protein only when phosphorylated at S209.

BiowMW :24kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Casein Kinase 2 beta (phospho S209); Casein Kinase 2 beta (p-S209); p-CSK2B(Ser209); phospho-CSK2B(Ser209); Casein kinase 2 beta polypeptide; casein kinase IIβ; Casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSK2B_HUMAN; CSNK 2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a.

Immunogen :KLH conjugated synthesised phosphopeptide derived from human CK II beta around the phosphorylation site of Ser209:FK(p-S)PV

Conjugate :Unconjugated

Modification :Phosphorylation

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK II beta (Ser209)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK II beta (Ser209)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.