Primary: Anti- PPHLN1 at 1/1000 dilution
Primary: Anti- PPHLN1 at 1/1000 dilution
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.
