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MEK-3 (phospho Thr222) Polyclonal Antibody

货号: BS65371

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS65371

Product Name :MEK-3 (phospho Thr222) Polyclonal Antibody

Swiss-Prot :P46734

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB,IHC-p,IF(paraffin section),ELISA

Application_all :Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Background :mitogen-activated protein kinase kinase 3(MAP2K3) Homo sapiens The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008],

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phospho-MEK-3 (T222) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at T222.

BiowMW :~39 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :MAP2K3; MEK3; MKK3; PRKMK3; SKK2; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; SAPKK-2; SAPKK2

Immunogen :The antiserum was produced against synthesized peptide derived from human MAP2K3 around the phosphorylation site of Thr222. AA range:188-237

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of lysates from Jurkat cells treated with serum 20% 15', using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using MAP2K3 (Phospho-Thr222) Antibody. The picture on the right is blocked with the phospho peptide.