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MDM2 (phospho Ser186/S188) polyclonal antibody

货号: BS65101

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS65101

Product Name :MDM2 (phospho Ser186/S188) polyclonal antibody

Swiss-Prot :Q00987

Host :Rabbit

Reactivity :Human

Applications :IF/ICC,WB,ELISA

Application_all :WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Background :MDM2 proto-oncogene(MDM2) Homo sapiens This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phospho-MDM2 (S186/S188) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S186/S188.

BiowMW :/

Note :For research use only, not for use in diagnostic procedure.

Pathway :Examples of Crosstalk Between Post-translational Modifications,

Alternative Name :MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2

Immunogen :The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. AA range:151-200

Conjugate :Unconjugated

Modification :Phosphorylation

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MDM2 (Phospho-Ser186+Ser188) Antibody

Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) Antibody. The picture on the right is blocked with the phospho peptide.