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[KO Validated] RAG2 polyclonal antibody

货号: BS65018

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS65018

Product Name :[KO Validated] RAG2 polyclonal antibody

Swiss-Prot :P55895

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB,IF

Application_all :WB 1:500 - 1:2000 IF 1:50 - 1:200

Background :This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RAG2 polyclonal antibody detects endogenous levels of RAG2 protein.

BiowMW :~ 59 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAG2; RAG-2

Immunogen :Recombinant fusion protein containing a sequence corresponding to amino acids 258-527 of human RAG2

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using RAG2 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and RAG2 Rabbit pAb knockout (KO) 293T cells, using RAG2 Rabbit pAb antibody at 1:500 dilution.

Immunofluorescence analysis of U2OS cells using RAG2 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using RAG2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.