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Bak polyclonal antibody

货号: BS6477

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6477

Product Name :Bak polyclonal antibody

Swiss-Prot :Q16611

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC

Application_all :WB 1:500 - 1:2000 IHC 1:50 - 1:100

Background :Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Bak polyclonal antibody detects endogenous levels of BAK1 protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7; BAK1; BAK; BCL2L7; CDN1

Immunogen :Recombinant full length Human Bak.

Conjugate :Unconjugated

Modification :Unmodification

WesternBlot (WB) analysis of Bak polyclonal antibody in extracts from OVCAR8, Jurkat, THP1 and 7901 cells.

Immunohistochemistry (IHC) analysis of Bak pAb in paraffin-embedded Human Stomach Cancer tissue.

Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through Fas- and mitochondria-mediated pathways

JournalMolecular Carcinogenesis

ApplicationWB

IF4.784

SpeciesHuman

PMID22821714
Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells

JournalPLoS One

ApplicationWB

IF3.24

SpeciesHuman

PMID24098511
Silencing Prion Protein in MDA-MB-435 Breast Cancer Cells Leads to Pleiotropic Cellular Responses to Cytotoxic Stimuli

JournalPLoS One

ApplicationWB

IF2.776

SpeciesHuman

PMID23133614
Curcumin induces p53-independent necrosis in H1299 cells via a mitochondria-associated pathway

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID26460892