产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

TORC2 (Phospho-S171) polyclonal antibody

货号: BS64579

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64579

Product Name :TORC2 (Phospho-S171) polyclonal antibody

Swiss-Prot :Q53ET0

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling. The protein CRTC2 (CREB-regulated transcription coactivator 2)/TORC2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways. In quiescent cells, CRTC2/TORC2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of CRTC2/TORC2 and its dissociation from 14-3-3 proteins. Dephosphorylated CRTC2/TORC2 enters the nucleus to promote CREB-dependent transcription. CRTC2/TORC2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. CRTC2/TORC2-related proteins CRTC1/TORC1 and CRTC3/TORC3 also act as CREB co-activators. CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3 associate with the HTLV Tax protein to promote Tax-dependent transcription of HTLV-1 long terminal repeats. CRTC1/TORC1 is highly phosphorylated at Ser151 in mouse hypothalamic cells under basal conditions. When these cells are exposed to cAMP or a calcium activator, CRTC1/TORC1 is dephosphorylated and translocates into the nucleus. CRTC1/TORC1 is essential for energy balance and fertility.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :TORC2 (Phospho-S171) polyclonal antibody detects endogenous levels of TORC2 protein only when phosphorylated at Ser171.

BiowMW :~ 87, 73 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2; CRTC2; TORC2

Immunogen :Synthetic phosphopeptide derived from human TORC2 around the phosphorylation site of Serine 171.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of TORC2 (Phospho-S171) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HEK293T whole cell lysate

Schistosoma japonicum infection causes a reprogramming of glycolipid metabolism in the liver

JournalParasites & Vectors

ApplicationWB

IF2.824

SpeciesMouse

PMID31375125