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LAT (Phospho-Y161) polyclonal antibody

货号: BS64562

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS64562

Product Name :LAT (Phospho-Y161) polyclonal antibody

Swiss-Prot :O43561

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LAT (Phospho-Y161) polyclonal antibody detects endogenous levels of LAT protein only when phosphorylated at Tyr161.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT

Immunogen :Synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyrosine 161.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of LAT (Phospho-Y161) polyclonal antibody at 1:500 dilution LaneA:The Heart tissue lysate of Rat LaneB:The Lung tissue lysate of Rat LaneC:The Heart tissue lysate of Mouse LaneD:The Lung tissue lysate of Mouse LaneE:HEK293T whole cell lysate LaneF:Jurkat whole cell lysate