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WEE1 (Phospho-S642) polyclonal antibody

货号: BS64542

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64542

Product Name :WEE1 (Phospho-S642) polyclonal antibody

Swiss-Prot :P30291

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB:1:500~1:1000

Background :Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1. Wee1 is inactivated upon mitotic entry by phosphorylation at Ser53 and Ser123 by Plk1 and cdc2, followed by beta-TrCP-mediated ubiquitination and degradation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :WEE1 (Phospho-S642) polyclonal antibody detects endogenous levels of WEE1 protein only when phosphorylated at Ser642.

BiowMW :~ 72 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Wee1-like protein kinase; WEE1hu; Wee1A kinase; WEE1

Immunogen :Synthetic phosphopeptide derived from human WEE1 around the phosphorylation site of Serine 642.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of WEE1 (Phospho-S642) polyclonal antibody at 1:500 dilution LaneA:The Brain tissue lysate of Mouse LaneB:The Brain tissue lysate of Rat