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Histone Deacetylase 3 (Phospho-S424) polyclonal antibody

货号: BS64528

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS64528

Product Name :Histone Deacetylase 3 (Phospho-S424) polyclonal antibody

Swiss-Prot :O15379

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background :In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Histone Deacetylase 3 (Phospho-S424) polyclonal antibody detects endogenous levels of Histone Deacetylase 3 protein only when phosphorylated at Ser424.

BiowMW :~ 49 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Notch Signaling,Nuclear Receptor Signaling,Epigenetic Writers and Erasers of Histone H3,

Alternative Name :Histone deacetylase 3; HD3; RPD3-2; SMAP45; HDAC3

Immunogen :Synthetic phosphopeptide derived from human Histone Deacetylase 3 around the phosphorylation site of Serine 424.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Histone Deacetylase 3 (Phospho-S424) polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:HGC27 whole cell lysate