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MK2 (Phospho-T222) polyclonal antibody

货号: BS64510

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS64510

Product Name :MK2 (Phospho-T222) polyclonal antibody

Swiss-Prot :P49137

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC:1:50~1:200

Background :In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MK2 (Phospho-T222) polyclonal antibody detects endogenous levels of MK2 protein only when phosphorylated at Thr222.

BiowMW :~ 49 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Microtubule Dynamics,Signaling Pathways Activating p38 MAP Kinase

Alternative Name :MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2

Immunogen :Synthetic phosphopeptide derived from human MK2 around the phosphorylation site of Threonine 222.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of MK2 (Phospho-T222) polyclonal antibody at 1:500 dilution LaneA:U-87MG whole cell lysate LaneB:K562 whole cell lysate LaneC:The Liver tissue lysate of Mouse LaneD:The Liver tissue lysate of Rat