产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Cyclin B1 (Phospho-S147) polyclonal antibody

货号: BS64509

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64509

Product Name :Cyclin B1 (Phospho-S147) polyclonal antibody

Swiss-Prot :P14635

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background :In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13 SUC1). The Cdc/cyclin enzyme is subject to multiple levels of control of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has estalished that B-type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cyclin B1 (Phospho-S147) polyclonal antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at Ser147.

BiowMW :~ 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :CCNB1; CCNB; cyclin B1; G2/mitotic-specific cyclin-B1

Immunogen :Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Cyclin B1 (Phospho-S147) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:Hela whole cell lysate LaneC:The Testis tissue lysate of Mouse LaneD:The Testis tissue lysate of Rat