Product Name :AKT (Phospho-Y474) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB:1:500~1:1000 IHC:1:50~1:200
Background :AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :AKT (Phospho-Y474) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Tyr474.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,Protein Kinase C Signaling,Immune Cell Signaling,Warburg Effect,Insulin &Glucose Signaling,ErbB HER Signaling,
Alternative Name :RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC
Immunogen :Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Tyrosine 474.
Modification :Phosphorylation
