产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

RRN3 (phospho-S649) polyclonal antibody

货号: BS64367

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64367

Product Name :RRN3 (phospho-S649) polyclonal antibody

Swiss-Prot :Q9NYV6

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :In eukaryotes, ribosomal RNA genes are transcribed by RNA polymerase (pol I). In Saccharomyces cerevisiae, transcription of rRNA genes requires at least three transcription factors, which include the two multisubunit factors Core factor and UAF that function in the assembly of the preinitiation complex. The third factor, Rrn3, functions as a single subunit and is not required for the preinitiation complex assembly. Unlike other pol I transcription factors, Rrn3 is functionally conserved between yeast and mammals as an rRNA gene transcription regulator. Human Rrn3 is 21% homologous to the yeast Rrn3 protein and is a member of a conserved gene family spanning the fungi, plant and animal kingdoms. hRrn3 is highly expressed in the lung, retina, thymus, and prostate. Rrn3 may be identical to the transcription factor TIF-IA, since both TIF-IA and Rrn3 associate with pol I and their activities are growth rate dependent.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RRN3 (phospho-S649) polyclonal antibody detects endogenous levels of RRN3 protein only when phosphorylated at Ser649 .

BiowMW :~ 80 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RNA polymerase I-specific transcription initiation factor RRN3; Transcription initiation factor IA; TIF-IA; RRN3; TIFIA

Immunogen :Synthetic phosphopeptide derived from human RRN3 around the phosphorylation site of Serine649.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of RRN3 (phospho-S649) polyclonal antibody at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:EC9706 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug)