产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

RAF1 (phospho-S289) polyclonal antibody

货号: BS64329

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64329

Product Name :RAF1 (phospho-S289) polyclonal antibody

Swiss-Prot :P04049

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Raf-1 is an ubiquitously expressed cytoplasmic protein with intrinsic serine/ threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RAF1 (phospho-S289) polyclonal antibody detects endogenous levels of RAF1 protein only when phosphorylated at Ser289 .

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Inhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,hippo Signaling,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF

Immunogen :Synthetic phosphopeptide derived from human RAF1 around the phosphorylation site of Serine289.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of RAF1 (phospho-S289) polyclonal antibody at 1:500 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)