产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKD1 (phospho-S205) polyclonal antibody

货号: BS64246

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS64246

Product Name :PKD1 (phospho-S205) polyclonal antibody

Swiss-Prot :Q15139

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PRKD1(Phospho-S205) polyclonal antibody detects endogenous levels of PRKD1 protein only when phosphorylated at Ser205.

BiowMW :~ 115 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu; PRKD1; PKD; PKD1; PRKCM

Immunogen :Synthetic phosphopeptide derived from human PRKD1 around the phosphorylation site of Serine 205.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of PRKD1(Phospho-S205) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with PMA(50ng/ml,10 minutes) whole cell lysate Lane3:HEK293T treated with PMA(50ng/ml,30 minutes) whole cell lysate Lane4:H9C2 whole cell lysate Lane5:3T3-L1 whole cell lysate