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PGR (phospho-S400) polyclonal antibody

货号: BS64224

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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No. :BS64224

Product Name :PGR (phospho-S400) polyclonal antibody

Swiss-Prot :P06401

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B. Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription. The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms. Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2. Mutation of Ser190 results in decreased activity of PR, suggesting that the phosphorylation at Ser190 may be critical to its biological function.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PGR(Phospho-S400) polyclonal antibody detects endogenous levels of PGR protein only when phosphorylated at Ser400.

BiowMW :~ 98 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,

Alternative Name :Progesterone receptor; PR; Nuclear receptor subfamily 3 group C member 3; PGR; NR3C3

Immunogen :Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 400.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of PGR(Phospho-S400) polyclonal antibody at 1:500 dilution Lane1:The Testis tissue lysate of Mouse(40ug) Lane2:The Ovary tissue lysate of Rat(40ug) Lane3:Hela whole cell lysate(30ug)