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Phospho-RB-S795 polyclonal antibody

货号: BS6414

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS6414

Product Name :Phospho-RB-S795 polyclonal antibody

Swiss-Prot :P06400

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:200

Background :The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :106kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :OSRC;PPP1R130;RB;p105-Rb;pRb;pp110;RB1;Rb

Immunogen :A synthetic phosphorylated peptide around S795 of human RB(NP_000312.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of 293 cell line, using phospho-RB-S795 antibody .
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Western blot analysis of extracts of K-562 cells, using Phospho-RB-S795 pAb at 1:1000 dilution or RB1 antibody .K-562 cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-RB-S795 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse lung using Phospho-RB-S795 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Oxidized ATM promotes abnormal proliferation of breast CAFs through maintaining intracellular redox homeostasis and activating the PI3K-AKT, MEK-ERK, and Wnt-β-catenin signaling pathways

JournalCell Cycle

ApplicationWB

IF3.699

SpeciesHuman

PMID25970706
Autocrine TGF-β1/miR-200s/miR-221/DNMT3B regulatory loop maintains CAF status to fuel breast cancer cell proliferation

JournalCancer Letters

ApplicationWB

IF7.36

SpeciesHuman

PMID30851420