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RASGRF1(Phospho-S916) polyclonal antibody

货号: BS64139

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS64139

Product Name :RASGRF1(Phospho-S916) polyclonal antibody

Swiss-Prot :Q13972

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate-limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF p140. Ras-GRF accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins. Of interest, a region mapping within the amino terminal domain of Ras-GRF is similar to both the human breakpoint cluster protein, Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasing factor for CDC42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RASGRF1(Phospho-S916) polyclonal antibody detects endogenous levels of RASGRF1 protein only when phosphorylated at Ser916.

BiowMW :~ 145 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25; RASGRF1; CDC25; GNRP; GRF1

Immunogen :Synthetic phosphopeptide derived from human RASGRF1 around the phosphorylation site of Serine 916.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of RASGRF1(Phospho-S916) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug)