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Phospho-Raf1-S621 polyclonal antibody

货号: BS6413

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

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No. :BS6413

Product Name :Phospho-Raf1-S621 polyclonal antibody

Swiss-Prot :P04049

Host :Rabbit

Reactivity :Human

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :73kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CMD1NN;CRAF;NS5;Raf-1;c-Raf;RAF1

Immunogen :A synthetic phosphorylated peptide around S621 of human Phospho-Raf1-S621(NP_002871.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa and 293T cells, using Phospho-Raf1-S621 antibody at 1:1000 dilution. HeLa cells were treated by EGF for 30 minutes after serum-starvation overnight. 293T cells were treated by PMA/TPA for 30 minutes or treated by EGF for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-Raf1-S621 pAb . Western blot was performed from the immunoprecipitate using Phospho-Raf1-S621 pAb at a dilution of 1:1000. HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.