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Phospho-Rac1/2/3-S71 polyclonal antibody

货号: BS6412

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6412

Product Name :Phospho-Rac1/2/3-S71 polyclonal antibody

Swiss-Prot :P63000

Host :Rabbit

Reactivity :Human, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :28KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RAC1;MIG5;Rac-1;TC-25;p21-Rac1

Immunogen :A synthetic phosphorylated peptide around S71 of human RAC1(NP_008839.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of A-431 cells, using Phospho-Rac1/2/3-S71 antibody at 1:1000 dilution.A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 180s.