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SMAD3 (phospho-S423) polyclonal antibody

货号: BS64037

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS64037

Product Name :SMAD3 (phospho-S423) polyclonal antibody

Swiss-Prot :P84022

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFß/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP2 and BMP4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFß and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFß signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SMAD3(p-s423) polyclonal antibody detects endogenous levels of SMAD3 protein only when phosphorylated at ser423.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Immunogen :A synthetic peptide corresponding to residues in Human SMAD3 around the phosphorylation site of Ser423.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-SMAD3 (S423) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with EGF(0.1ng/ML,30mins) Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ML,30mins) Lane3:H9C2 cell lysate treated with EGF(0.1ng/ML,30mins)

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