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Phospho-MET-Y1349 polyclonal antibody

货号: BS6403

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS6403

Product Name :Phospho-MET-Y1349 polyclonal antibody

Swiss-Prot :P08581

Host :Rabbit

Reactivity :Human, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :160kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MET;AUTS9;DFNB97;HGFR;RCCP2;c-Met

Immunogen :A synthetic phosphorylated peptide around Y1349 of human MET(NP_000236.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1349 antibody at 1:1000 dilution. HeLa cells were treated by HGF for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 90s.