Product Name :PLC γ2 (phospho-Y759) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
Background :Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptormediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol fromphosphatidylinositol 4,5-bisphosphate.There are manymammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associateswith several tyrosine-phosphorylated proteins(Syk, SLP-76,Lyn, linker for activation of T cells(LAT) and the FcR γ chain),which bind to its C-terminal SH2 domain. PLC γ1 associateswith Syk in B cells, but PLC γ2 does not associatewith Sykin platelets. The C-terminal SH2 domain isinvolved in the regulation of PLC γ2. In addition, Btkcan induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-
stimulated B lymphocytes.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-PLC γ2 (Y759) polyclonal antibody detects endogenous levels of PLC γ2 protein only when phosphorylated at Tyr759.
Note :For research use only, not for use in diagnostic procedure.
Pathway :B cell receptor signaling,
Alternative Name :1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; EC=3.1.4.11; Phosphoinositide phospholipase C-gamma-2 Phospholipase C-IV; PLC-IV; Phospholipase C-gamma-2; PLC-gamma-2
Immunogen :A synthetic peptide corresponding to residues in Human PLC γ2 around the phosphorylation site of Tyrosine 759.
Modification :Phosphorylation
