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Phospho-MAP2K4-S80 polyclonal antibody

货号: BS6393
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6393
Product Name :Phospho-MAP2K4-S80 polyclonal antibody
Swiss-Prot :P45985
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200
Background :This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Phosphorylated Antibodies
BiowMW :44kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :MAP2K4;JNKK;JNKK1;MAPKK4;MEK4;MKK4;PRKMK4;SAPKK-1;SAPKK1;SEK1;SERK1;SKK1
Immunogen :A synthetic phosphorylated peptide around S80 of human MAP2K4(NP_003001.1).
Conjugate :Unconjugated
Modification :Phosphorylated
Western blot analysis of extracts of NIH/3T3 and C6 cells, using Phospho-MAP2K4-S80 antibody at 1:1000 dilution. NIH/3T3 cells were treated by PDGF for 30 minutes after serum-starvation overnight or treated by PDGF for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 150s.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com