产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Phospho-MEK1-S298 polyclonal antibody

货号: BS6389

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6389

Product Name :Phospho-MEK1-S298 polyclonal antibody

Swiss-Prot :Q02750

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:100

Background :The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :43kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CFC3;MAPKK1;MEK1;MKK1;PRKMK1;MAP2K1

Immunogen :A synthetic phosphorylated peptide around S298 of human MEK1(NP_002746.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of various cell lines, using Phospho-MEK1-S298 antibody at 1:1000 dilution or MEK1 antibody . HeLa cells were treated by PMA/TPA at 37℃ for 15 minutes after serum-starvation overnight or treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using Phospho-MEK1-S298 antibody at 1:1000 dilution. C2C12 cells were treated by CIP at 37℃ for 1 hour or treated by ATP at 30℃ for 1 hour. C6 cells were treated by CIP at 37℃ for 1 hour or treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-MEK1-S298 pAb . Western blot was performed from the immunoprecipitate using Phospho-MEK1-S298 pAb at a dilution of 1:1000. 293T cells were treated by PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.