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Phospho-MAP3K5-S1033 polyclonal antibody

货号: BS6384

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS6384

Product Name :Phospho-MAP3K5-S1033 polyclonal antibody

Swiss-Prot :Q99683

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :155kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MAP3K5;ASK1;MAPKKK5;MEKK5

Immunogen :A synthetic phosphorylated peptide around S1033 of human MAP3K5(NP_005914.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody at 1:1000 dilution. A431 cells were treated by PMA/TPA . HeLa cells were treated by 10% FBS or treated by PMA/TPA for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.