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Phospho-GSK3β-S9 polyclonal antibody

货号: BS6365

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6365

Product Name :Phospho-GSK3β-S9 polyclonal antibody

Swiss-Prot :P49841

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :47KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :GSK3B;gsk-3β

Immunogen :A synthetic phosphorylated peptide around S9 of human GSK3β(NP_001139628.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of C6, using Phospho-GSK3β-S9 antibody at 1:545 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using Phospho-GSK3β-S9 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Phospho-GSK3β-S9 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Phospho-GSK3β-S9 antibody . Western blot was performed from the immunoprecipitate using Phospho-GSK3β-S9 antibody at a dilution of 1:1000.