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Phospho-eIF4EBP1-T70 polyclonal antibody

货号: BS6357

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS6357

Product Name :Phospho-eIF4EBP1-T70 polyclonal antibody

Swiss-Prot :Q13541

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :17kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :4E-BP1;4EBP1;BP-1;PHAS-I;EIF4EBP1

Immunogen :A synthetic phosphorylated peptide around T70 of human Phospho-eIF4EBP1-T70(NP_004086.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-eIF4EBP1-T70 antibody at 1:2000 dilution. HeLa cells were treated by Insulin at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit .
Exposure time: 3min.