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Phospho-EGFR-Y1148 polyclonal antibody

货号: BS6354

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6354

Product Name :Phospho-EGFR-Y1148 polyclonal antibody

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :180kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA

Immunogen :A synthetic phosphorylated peptide around Y1148 of human EGFR(NP_005219.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1148 antibody at 1:1000 dilution. A431 cells were treated by EGF for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.