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Phospho-CREB1-S133 polyclonal antibody

货号: BS6345

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6345

Product Name :Phospho-CREB1-S133 polyclonal antibody

Swiss-Prot :P16220

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:200 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:100

Background :This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :43kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CREB1;CREB;CREB-1

Immunogen :A synthetic phosphorylated peptide around S133 of human CREB1(NP_604391.1).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of HeLa cells, using Phospho-CREB1-S133 pAb at 1:2000 dilution or CREB1 antibody .HeLa cells were treated by Anisomycin at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-CREB1-S133 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CREB1-S133 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat kidney using Phospho-CREB1-S133 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

The Involvement of NR2B and tau Protein in MG132-Induced CREB Dephosphorylation

JournalJournal Of Molecular Neuroscience

ApplicationWB

IF2.577

SpeciesHuman

PMID28424976