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Phospho-B-Raf-S445 polyclonal antibody

货号: BS6338
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6338
Product Name :Phospho-B-Raf-S445 polyclonal antibody
Swiss-Prot :P15056
Host :Rabbit
Reactivity :Human, Rat
Applications :WB
Application_all :WB,1:500 - 1:2000
Background :This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Phosphorylated Antibodies
BiowMW :100kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Immunogen :A synthetic phosphorylated peptide around S445 of human BRAF(NP_004324.2).
Conjugate :Unconjugated
Modification :Phosphorylated
Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb at 1:2000 dilution or BRAF antibody .Raji cells were treated by PMA/TPA for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 10s.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com