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Phospho-AKT1-T450 polyclonal antibody

货号: BS6330

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS6330

Product Name :Phospho-AKT1-T450 polyclonal antibody

Swiss-Prot :P31749

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:1000

Background :The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Phosphorylated Antibodies

BiowMW :60kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1

Immunogen :A synthetic phosphorylated peptide around T450 of human Akt1(NP_005154.2).

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of extracts of 293 and HeLa cells, using Phospho-AKT1-T450 antibody at 1:1000 dilution. 293 cells were treated by Insulin for 10 minutes after serum-starvation overnight. HeLa cells were treated by EGF for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit .
Exposure time: 1min.