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p116 Rip polyclonal antibody

货号: BS62404

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS62404

Product Name :p116 Rip polyclonal antibody

Swiss-Prot :Q6WCQ1

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC:1:50~1:200

Background :Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Purification&Purity :The protein was purified from E.coli and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p116 Rip polyclonal antibody detects endogenous levels of p116 Rip protein.

BiowMW :~ 116 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Myosin phosphatase Rho-interacting protein; M-RIP; Rho-interacting protein 3; RIP3; p116Rip; MPRIP

Immunogen :Synthetic peptide, corresponding to Human p116 Rip.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of p116 Rip polyclonal antibody at 1:500 dilution LaneA:PC12 whole cell lysate LaneB:MEF whole cell lysate LaneC:A549 whole cell lysate LaneD:A2780 whole cell lysate