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PKC alpha polyclonal antibody

货号: BS62271

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS62271

Product Name :PKC alpha polyclonal antibody

Swiss-Prot :P17252

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC:1:50~1:200

Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKC polyclonal antibody detects endogenous levels of PKC protein.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phospholipase Signaling,Protein Kinase C Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA

Immunogen :Synthetic peptide, corresponding to Human PKC.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PKC alpha polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:COS-7 whole cell lysate LaneC:HEK293T whole cell lysate LaneD:C6 whole cell lysate LaneE:CT26 whole cell lysate

Recombinant Human Angiopoietin-1 Ameliorates the Expressions of ZO-1, Occludin, VE-cadherin, and PKCα Signaling after Focal Cerebral Ischemia/Reperfusion in Rats

JournalJournal Of Molecular Neuroscience

ApplicationWB

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SpeciesRat

PMID21710361
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ApplicationWB

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