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MYT1 polyclonal antibody

货号: BS62249

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS62249

Product Name :MYT1 polyclonal antibody

Swiss-Prot :Q99640

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC:1:50~1:200

Background :Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MYT1 polyclonal antibody detects endogenous levels of MYT1 protein.

BiowMW :~ 70, 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1

Immunogen :Synthetic peptide, corresponding to Human MYT1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MYT1 polyclonal antibody at 1:500 dilution LaneA:The Liver tissue lysate of Rat LaneB:The Muscle tissue lysate of Rat

免疫沉淀（IP）

免疫组织化学（IHC）

免疫荧光（IF）