产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PAPOLA polyclonal antibody

货号: BS61818

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS61818

Product Name :PAPOLA polyclonal antibody

Swiss-Prot :P51003

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Polyadenylation of the 3' ends of eukaryotic mRNAs is a key event that takes place in the nucleus during maturation of mRNA. The reaction occurs in two distinct steps: endoribonucleolytic cleavage of the pre-RNA at the poly(A) site, followed by synthesis of the poly(A) tail at the 3' end of the up-stream cleavage product. The poly(A) polymerase (PAP) is required for the adenosine addition reaction. Western blot analysis reveals three PAPs, namely PAP-α, PAP-β and PAP-γ, demonstrating different molecular masses in HeLa cell extracts. The amino-terminal region of PAP is required for nonspecific polymerase activity, while both the amino and carboxy termini are required for specific polymerase activity. Additionally, PAP contains a functional ribonucleoprotein-type RNA binding domain (RBD) that is responsible for primer binding.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PAPOLA pAb detects endogenous levels of PAPOLA protein.

BiowMW :~ 100 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Poly(A) polymerase alpha; PAP-alpha; Polynucleotide adenylyltransferase alpha; PAPOLA; PAP

Immunogen :Synthetic peptide, corresponding to Human PAPOLA.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PAPOLA pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate(40ug) Lane2:The Embryo tissue lysate of Mouse(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug)