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RAP2B polyclonal antibody

货号: BS61644

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS61644

Product Name :RAP2B polyclonal antibody

Swiss-Prot :P61225

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture. Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative “effector” domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity. Rap 2, another Ras-related protein, shares 60% identity with Rap 1A and exhibits a carboxy-terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1A and Rap 1B, overexpression of Rap 2 does not interfere with the Ras signaling pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RAP2B polyclonal antibody detects endogenous levels of RAP2B protein.

BiowMW :~ 21 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Ras-related protein Rap-2b; RAP2B

Immunogen :Synthetic peptide, corresponding Human RAP2B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of RAP2B polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:THP-1 whole cell lysate(40ug) Lane4:BV2 whole cell lysate(40ug) Lane5:PMVEC whole cell lysate(40ug)