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NOX4 polyclonal antibody

货号: BS60435

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60435

Product Name :NOX4 polyclonal antibody

Swiss-Prot :Q9NPH5

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NOX4 polyclonal antibody detects endogenous levels of NOX4 protein.

BiowMW :~ 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NADPH oxidase 4; Kidney oxidase-1; KOX-1; KOX1; Kidney superoxide-producing NADPH oxidase; Renal NAD(P)H-oxidase; NOX4; RENOX

Immunogen :A synthetic peptide corresponding to residues in Human NOX4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NOX4 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Ginsenoside Rg1 treatment alleviates renal fibrosis by inhibiting the NOX4–MAPK pathway in T2DM mice

JournalRENAL FAILURE

ApplicationWB

IF3.222

SpeciesMouse,Human

PMID37017270