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Nephrocystin-1 polyclonal antibody

货号: BS60189

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS60189

Product Name :Nephrocystin-1 polyclonal antibody

Swiss-Prot :O15259

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Nephrocystin-1 polyclonal antibody detects endogenous levels of Nephrocystin-1 protein.

BiowMW :~ 83 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nephrocystin1; Nephrocystin 1; Juvenile nephronophthisis 1 protein; NPHP1; NPH1

Immunogen :A synthetic peptide corresponding to residues in Human Nephrocystin-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Nephrocystin-1 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate