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WT1-AS polyclonal antibody

货号: BS60050

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS60050

Product Name :WT1-AS polyclonal antibody

Swiss-Prot :Q06250

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Wilms’ tumor is a neoplasm of the kidneys that usually occurs in children and is characterized by the presence of abortive tubules and glomeruli surrounded by a spindled cell stroma. The 11p13 Wilms’ tumor locus consists of two coordinately regulated transcripts, WT1 and WIT-1, which are mutated in Wilms’ tumors. Wilms’ tumor upstream neighbor 1 (WIT-1) is encoded by an intron-less gene upstream of the Wilms’ tumor 1 (WT1) gene, which is is important for nephrogenesis and gonadal growth. The WT1 gene is bi-directionally transcribed from the same promoter region as WIT-1, which may function as an antisense regulator of WT1. WIT-1 and WT1 have the same temporal and cell-restricted expression pattern, although the expression of WIT-1 is less abundant. Methylation of the WIT-1 gene is implicated in hematologic malignancy of chemoresistant acute myeloid leukemia. Single nucleotide polymorphisms (SNPs) in the WIT-1 gene are significantly associated with focal segmental glomerulosclerosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :WT1-AS polyclonal antibody detects endogenous levels of WT1-AS protein.

BiowMW :~ 15 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Putative Wilms tumor upstream neighbor 1 gene protein; WIT-1; Wilms tumor-associated antisense RNA; WT1-AS; WIT1

Immunogen :A synthetic peptide corresponding to residues in Human WT1-AS.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of WT1-AS polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate