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SPARC polyclonal antibody

货号: BS60017

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS60017

Product Name :SPARC polyclonal antibody

Swiss-Prot :P09486

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SPARC polyclonal antibody detects endogenous levels of SPARC protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :SPARC; Basement-membrane protein 40; BM-40; Osteonectin; Short name=ON; Secreted protein acidic and rich in cysteine; SPARC; ON

Immunogen :A synthetic peptide corresponding to residues in Human SPARC.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SPARC polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:PC12 whole cell lysate