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ZC3H8 polyclonal antibody（KU）

货号: BS5770

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS5770

Product Name :ZC3H8 polyclonal antibody（KU）

Swiss-Prot :Q8N5P1

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Zinc-finger CCCH domain-containing protein 8 (ZC3H8) acts as a transcriptional repressor of the GATA3 promoter. It is a sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene (by similarity). ZC3H8 induces thymocyte apoptosis when overexpressed, which may indicate a role in regulation of thymocyte homeostasis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ZC3H8 polyclonal antibody detects endogenous levels of ZC3H8 protein.

BiowMW :~ 33 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Zinc finger CCCH domain-containing protein 8; ZC3HDC8

Immunogen :Synthetic peptide, corresponding to amino acids 133-178 of Human ZC3H8.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of ZC3H8 pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(20ug) Lane2:Hela whole cell lysate(20ug) Lane3:C6 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of ZC3H8 pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.