Product Name :IRS-1 (P618) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
Background :IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :IRS-1 (P618) polyclonal antibody detects endogenous levels of IRS-1 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,
Alternative Name :Insulin receptor substrate 1; IRS-1; IRS1
Immunogen :Synthetic peptide, corresponding to amino acids 600-650 of Human IRS-1.
Modification :Unmodification
