Product Name :Cofilin 1 (phospho-S3) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
Background :Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-COFILN1 (S3) polyclonal antibody detects endogenous levels of COFILN1 protein only when phosphorylated at Ser3.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,Regulation of Actin Dynamics,Tumor Angiogenesis,Regulation of Microtubule Dynamics,B cell receptor signaling,
Alternative Name :Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL
Immunogen :Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.
Modification :Phosphorylation
