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Cdk1/Cdc2 (phospho-Y15) polyclonal antibody

货号: BS5058

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS5058

Product Name :Cdk1/Cdc2 (phospho-Y15) polyclonal antibody

Swiss-Prot :P06493

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Cdk1/Cdc2 (Y15) polyclonal antibody detects endogenous levels of Cdk1/Cdc2 protein only when phosphorylated at Tyr15.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :Cyclin-dependent kinase 1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase; CDK1; CDC2; CDC28A; CDKN1; P34CDC2

Immunogen :Synthetic phosphopeptide derived from human Cdk1/Cdc2 around the phosphorylation site of Serine 795.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Cdc2 (phospho-Y15) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Hela treated with UV (10min) whole cell lysate(40ug) Lane3:Hela treated with UV (20min) whole cell lysate(40ug) Lane4:Hela treated with UV (30min) whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Cdk1/Cdc2 (Y15) pAb in paraffin-embedded human breast carcinoma tissue.