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SREBP-1 (phospho-S439) polyclonal antibody（KU）

货号: BS4912

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4912

Product Name :SREBP-1 (phospho-S439) polyclonal antibody（KU）

Swiss-Prot :P36956

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. SREBP-1 (also designated ADD1, for adipocyte determination and differentiation factor) is synthesized as a precursor that is attached to the nuclear envelope and endoplasmic reticulum. In sterol-depleted cells, the membrane-bound precursor is cleaved to generate a soluble NH2-terminal fragment that translocates to the nucleus to activate transcription. Sterols inhibit the cleavage of SREBP-1.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SREBP-1 (phospho-S439) polyclonal antibody detects endogenous levels of SREBP-1 protein only when phosphorylated at Ser439.

BiowMW :~ 121 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1; Processed sterol regulatory element-binding protein 1; SREBF1; BHLHD1; SREBP1

Immunogen :Synthetic phosphopeptide derived from human SREBP-1 around the phosphorylation site of Serine 439

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of SREBP-1 (phospho-S439) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Mark4 promotes adipogenesis and triggers apoptosis in 3T3-L1 adipocytes by activating JNK1 and inhibiting p38MAPK pathways

JournalBiology Of The Cell

ApplicationWB

IF4.458

SpeciesMouse

PMID