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MAPKAPK-2 (phospho-S272) polyclonal antibody（KU）

货号: BS4902

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4902

Product Name :MAPKAPK-2 (phospho-S272) polyclonal antibody（KU）

Swiss-Prot :P49137

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.

Product :1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MAPKAPK-2 (phospho-S272) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Ser272.

BiowMW :~ 47, 49 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Microtubule Dynamics,Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway,

Alternative Name :MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2; MAPKAPK2

Immunogen :Synthetic phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of Serine 272

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of MAPKAPK-2 (phospho-S272) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Spleen tissue lysate of Mouse(40ug) Lane3:The Spleen tissue lysate of Rat(40ug)