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ZAP70 (phospho-Y315) polyclonal antibody

货号: BS4884

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4884

Product Name :ZAP70 (phospho-Y315) polyclonal antibody

Swiss-Prot :P43403

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes . The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer . The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains . This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 . ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes . Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 .

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-ZAP70 (Y315) polyclonal antibody detects endogenous levels of ZAP70 protein only when phosphorylated at Tyr315.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,T cell receptor signaling,

Alternative Name :Tyrosine-protein kinase ZAP-70; 70 kDa zeta-chain associated protein; Syk-related tyrosine kinase; ZAP70; SRK

Immunogen :Synthetic phosphopeptide derived from human ZAP70 around the phosphorylation site of Tyrosine 315.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-ZAP70 (Y315) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with H2O2(100µM,15mins) Lane2:Mouse kidney tissue lysate Lane3:Rat kidney tissue lysate

CCL5 promotes LFA-1 expression in Th17 cells and induces LCK and ZAP70 activation in a mouse model of Parkinson’s disease

JournalFrontiers in Aging Neuroscience

ApplicationWB

IF4.8

SpeciesMouse

PMID38020765